ASSESSMENT OF POST-OPERATIVE PAIN AND ANTIBACTERIAL EFFECTIVENESS OF CHITOSAN NANOPARTICLES VERSUS SODIUM HYPOCHLORITE IRRIGATION IN INFECTED ROOT CANAL. (A RANDOMIZED CLINICAL TRIAL)

Research Abstract

Sarah El-Sayed Mahmoud Mahgoub; Assessment Of Post-operative Pain And Antibacterial Effectiveness Of Chitosan Nanoparticles Versus Sodium Hypochlorite Irrigation In Infected Root Canal. (A Randomized clinical trial) (Under the supervision of Siza Yacoub Zaghari, Shaimaa Gawdat, and Olfat Shaker) Aim: The aim of the study was to assess the bacterial reduction and postoperative pain of Chitosan nanoparticles (3% CNPs) versus Sodium hypochlorite (2.5% NaOCl) when used as a root canal irrigant during instrumentation after single visit endodontic treatment of patients with necrotic maxillary anterior teeth. Methodology: Twenty two patients with necrotic maxillary anterior teeth, were endodontically treated in a single visit. The patients were divided equally into two groups, A and B (n=11) according to the protocol of irrigation; Group A: Irrigation with Chitosan nanoparticles (3% CNPs); Group B: Irrigation with Sodium hypochlorite (2.5% NaOCl). Instrumentation was done using rotary ProTaper files in a crown down manner for all groups. During preparation canals were thoroughly irrigated using a total of 10 mL of irrigating solutions of the respective test group. After complete preparation, the canals were irrigated for 5 minutes using 5 ml of irrigating solutions of the respective test group as a final flush. For all groups manual dynamic agitation was done to activate the irrigating solution then canals where flushed with normal saline to stop any residual activity. Samples were collected from root canals immediately before preparation (S1) and after irrigation with the experimental solutions (S2) using sterilepaper points that were immediately transferred for microbiological processing. Root canal culture samples were analyzed by counting the colony forming units (CFUs) to evaluate the antimicrobial effectiveness of the proposed treatments. Results: There was no statistical significant difference in bacterial percentage reduction between the two groups. (p = 0.176). Regarding postoperative pain incidence and intensity at all follow-up intervals, there was no statistical significant difference between the two groups. Conclusions: Both of the irrigation protocols had efficient antibacterial effect against E. faecalis. Manually agitated chitosan nanoparticles (3% CNPs) can be utilized as an antibacterial irrigant in eradication of E. faecalis from root canal system and can be considered as a more safe and efficient alternative for sodium hypochlorite against E. faecalis. KEY WORDS: Nano-chitosan, Sodium hypochlorite, Enterococcus faecalis, Manual agitation, Root canal irrigants.

Research Keywords

KEY WORDS: Nano-chitosan, Sodium hypochlorite, Enterococcus faecalis, Manual agitation, Root canal irrigants.