Basic Informations

C.V

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Master Title

ASSESSMENT OF POST-OPERATIVE PAIN AND ANTIBACTERIAL EFFECTIVENESS OF CHITOSAN NANOPARTICLES VERSUS SODIUM HYPOCHLORITE IRRIGATION IN INFECTED ROOT CANAL. (A RANDOMIZED CLINICAL TRIAL)

Master Abstract

ENGLISH SUMMARY This randomized clinical trial was carried out to assess the bacterial reduction and post-operative pain of Chitosan nanoparticles (3% CNPs) when used as an endodontic irrigant versus sodium hypochlorite (2.5% NaOCl) during instrumentation in patients with necrotic maxillary anterior teeth after single-visit endodontic therapy. Twenty-two patients with necrotic maxillary anterior teeth were endodontically treated in a single visit. The patients were assigned equally into two groups, A and B (n = 11) according to the irrigation protocol; Group A: Irrigation with Chitosan nanoparticles (3% CNPs); Group B: Irrigation with Sodium hypochlorite (2.5% NaOCl). Mechanical preparation was done using ProTaper rotary files in a crown down manner for all groups. During preparation, the root canals were completely irrigated with a total of 10 mL of the appropriate test group's irrigating solution. After complete preparation, the canals were irrigated for 5 minutes using 5 ml of the respective test group's irrigating solutions as a final flush. For all groups, manual dynamic agitation was done to activate the irrigating solution, then the canals were flushed with normal saline to stop any residual activity. Root canal samples were collected immediately before mechanical instrumentation (S1) and after irrigation with the experimental solutions (S2) using sterile paper points that were immediately transferred for microbiological processing. Root canal culture samples were measured by counting the colony forming units (CFUs) to assess the antimicrobial efficacy of the suggested treatments. All demographic data, NRS scores obtained from the patients, and the count of CFU/ml for E. faecalis bacteria were subjected to statistical analysis. Our findings showed that there was no statistically significant difference in age, gender, or type of tooth between the two groups. The difference in bacterial reduction percentage between the two groups was not statistically significant (p = 0.176). Regarding postoperative pain incidence and intensity at all follow-up intervals, there was no statistically significant difference between the two groups. Within the limitations of the present study, it can be concluded that both of the irrigation protocols had an efficient antibacterial effect against E. faecalis. Manually agitated chitosan nanoparticles (3% CNPs) can be utilized as an antibacterial irrigant in the eradication of E. faecalis from root canal systems and can be considered as a more safe and efficient alternative to sodium hypochlorite against E. faecalis.

PHD Title

ASSESSMENT OF POST-OPERATIVE PAIN AND ANTIBACTERIAL EFFECTIVENESS OF CHITOSAN NANOPARTICLES VERSUS SODIUM HYPOCHLORITE IRRIGATION IN INFECTED ROOT CANAL. (A RANDOMIZED CLINICAL TRIAL)

PHD Abstract

ENGLISH SUMMARY This randomized clinical trial was carried out to assess the bacterial reduction and post-operative pain of Chitosan nanoparticles (3% CNPs) when used as an endodontic irrigant versus sodium hypochlorite (2.5% NaOCl) during instrumentation in patients with necrotic maxillary anterior teeth after single-visit endodontic therapy. Twenty-two patients with necrotic maxillary anterior teeth were endodontically treated in a single visit. The patients were assigned equally into two groups, A and B (n = 11) according to the irrigation protocol; Group A: Irrigation with Chitosan nanoparticles (3% CNPs); Group B: Irrigation with Sodium hypochlorite (2.5% NaOCl). Mechanical preparation was done using ProTaper rotary files in a crown down manner for all groups. During preparation, the root canals were completely irrigated with a total of 10 mL of the appropriate test group's irrigating solution. After complete preparation, the canals were irrigated for 5 minutes using 5 ml of the respective test group's irrigating solutions as a final flush. For all groups, manual dynamic agitation was done to activate the irrigating solution, then the canals were flushed with normal saline to stop any residual activity. Root canal samples were collected immediately before mechanical instrumentation (S1) and after irrigation with the experimental solutions (S2) using sterile paper points that were immediately transferred for microbiological processing. Root canal culture samples were measured by counting the colony forming units (CFUs) to assess the antimicrobial efficacy of the suggested treatments. All demographic data, NRS scores obtained from the patients, and the count of CFU/ml for E. faecalis bacteria were subjected to statistical analysis. Our findings showed that there was no statistically significant difference in age, gender, or type of tooth between the two groups. The difference in bacterial reduction percentage between the two groups was not statistically significant (p = 0.176). Regarding postoperative pain incidence and intensity at all follow-up intervals, there was no statistically significant difference between the two groups. Within the limitations of the present study, it can be concluded that both of the irrigation protocols had an efficient antibacterial effect against E. faecalis. Manually agitated chitosan nanoparticles (3% CNPs) can be utilized as an antibacterial irrigant in the eradication of E. faecalis from root canal systems and can be considered as a more safe and efficient alternative to sodium hypochlorite against E. faecalis.