Basic Informations

C.V

SARAH MOSTAFA SAYED MOHAMED Personal information • Birthday: 11/24/1990. • Address: Assuit- Manfalout –Bani Adi El-Wosta. • Mobile: 01119617417. • Email: Sara.mostafa@dent.bsu.edu.eg Education and Qualifications • Faculty of Dentistry -Minia university From: 2006 To:2011 Grades: Very good with high honors • Master's degree of Oral and Maxillofacial Pathology at Minia University, June 2017. EMPLOYMENT AND WORK EXPERIENCE: • General practitioner (dentist) at Assiut university hospitals. • From: January 2013 to April 2019. • Assistant lecturer at Faculty of Dentistry- Beni-Suef University. • From April 2019 till now. Language Skills • Arabic: native • English: very good (writing, speaking, understanding). • French, Korean, German and Turkish: good. Computer Skills • Microsoft Office: (good) • Word, PowerPoint, Excel. (Very Good) • Internet (good) Personal Skills • Good Communicator • Convincing • Team Leading • Work in Teamwork • Hard Worker • Intelligent • Friendly • Flexible Other / Personal Details Interests: Reading, Writing.

Master Title

Expression of Growth Factor Receptor Bounded Protein 2 and Human Epidermal Growth Factor 2 in Keratocystic Odontogenic Tumor (An Immunohistochemical Study)

Master Abstract

Summary The combination of clinically aggressive behavior, certain histopathological characteristics, and understanding of genetic nature changed the classification of what was known as an odontogenic keratocyst to keratocystic odontogenic tumor. Growth Factor Receptor-bound protein 2 (Grb2) is a key molecule in intracellular signal transduction, linking activated cell surface receptors to downstream targets (as it is located in the nucleus, cytoplasm, endosome, golgi apparatus) by binding to specific phosphotyrosine-containing and proline-rich sequence motifs. HER family includes four epidermal growth factor receptors, HER1, HER2, HER3, and HER4. They are also called as ErbB-1, ErbB-2, ErbB-3, and ErbB-4. These are transmembrane tyrosine kinase receptors and their functions include regulation of cell growth, cell survival, adhesion, migration, differentiation and cellular responses. The immunohistochemical results of the present study showed positive nuclear Her2 staining seen mainly in the suprabasal and granular layers of the epithelial lining of KCOTs. The immunohistochemical results of the present study showed that Grb2 immunoreactivity was seen in both cytoplasm and nucleus of the epithelial cells of KCOT. This Grb2 expression pattern might explain the neoplastic nature of KCOT as it was found that In human cancers, Grb2-mediated signaling (such as EGFR pathway) are often overexpressed, correlating with poor prognosis. Statistical analysis of the present study revealed that there is positive correlation between the HER2 and Grb2 expression in KCOT (R=0.931). The relationship between Grb2 and HER2 (erbB2) expression can be explained by the fact that Grb2 and HER2 are both located at chromosome 17q.

PHD Title

The Effect of Frankincense and Myrrh on Oral Squamous Cell Carcinoma Cell Line: An Ex-Vivo Study

PHD Abstract

Summary Oral squamous cell carcinoma (OSCC) is the world's most common neoplasm and the incidence of OSCC has increased in many countries and especially in young people. Surgery remains the first-line therapy option for OSCC. Its supported by radiotherapy (external beam radiation and brachytherapy) and chemotherapy (such as Fluorouracil (5-Fu) and cisplatin) or in combination. Alternative medicine and medicinal plants have been well accepted by the public with more than half of the worldwide population using them as a preventive or therapeutically effective anticancer drug (complementary, adjunctive, and alternative). Frankincense and Myrrh have medicinal properties, such as immunomodulatory, anti-inflammatory, cytotoxic, antioxidant, antimicrobial, hepatoprotective, anti-tumor, anti-ulcer, and analgesic activities. Consequently, Frankincense and Myrrh can be used to treat different types of diseases because of their therapeutic activities. Both Frankincense and Myrrh have modern pharmacological applications for several disease treatments, most of them predicted by the traditional therapies because of their unique chemical compositions, pharmacological activities. Non-toxicity tends to support the safe use of these popular traditional drugs in modern therapies. The synergistic effects of the combination formed by these two natural resins have been confirmed and have attracted worldwide attention. The studies evaluating Frankincense and Myrrh aqueous extract effects on oral squamous cell carcinoma are deficient. In this study, cell viability and cytotoxicity, caspase-3 and -8 activation, ROS activity, Mitochondrial transmembrane potential (??m), and cytological and morphological changes with nuclear area factor (NAF) calculation were evaluated and assessed to find out the likely mechanisms of cytotoxicity, growth inhibition, and apoptosis. In our research, the whole extract was used, unlike some previous searches, where the isolated compounds were used based on the concept that all is better than some and the components of the whole extract work in a synergistic manner. Frankincense and Myrrh different extracts have a cytotoxic effect and the aqueous extract was more effective than methanolic extract, the combination aqueous extract has the most cytotoxic and apoptotic effect than the other extracts and even more than 5-FU in SCC-25 cell lines. Frankincense, Myrrh, and combination aqueous extracts caused a large significant increase in ROS production. It also leads to loss of mitochondria membrane potential and a corresponding increase in caspase-3 and-8 activation. Therefore, this study suggested that Frankincense, Myrrh, and combination aqueous extracts cause oxidative stress through ROS-regulated activation, which causes the intrinsic and extrinsic apoptotic signaling cascades in SCC-25 cells. The cytonuclear morphological changes was convenient with the apoptotic, secondary necrotic, necrotic cell death morphology, and the reduction of NAF was seen in photomicrographed stained cells.