Basic Informations
C.V
Mohamed Osama Mostafa
Personal information
Education
- Doctor degree (PhD) in oral and maxillofacial pathology / Faculty of dentistry / Cairo University (2023-2024).
- Master of Oral Pathology/ Faculty of dentistry / Cairo University (2014).
- Internship for six months in faculty of oral and dental medicine departments, Cairo university and ministry of health (Al-Monera Hospital), (2007).
- Bachelor of Oral & Dental medicine / Cairo University / May 2006. Degree: very good.
Occupation and Experience:
- Lecturer of Oral Pathology – faculty of dentistry – Beni-Suef University. (2024)
- Lecturer assistant of Oral Pathology - faculty of dentistry - Beni-Suef University- Egypt (2016-2023).
- Lecturer assistant of Oral Pathology- faculty of dentistry – Ahram Canadian University – (part time) – (2019).
- Teaching assistant in Oral Pathology department, faculty of dentistry - Nahda University (2009 – 2015).
- Oral and maxillofacial pathologist in general pathology diagnostic lab (Vega Lab). (2021-now)
- Owner of private dental clinic (Royal dental clinic). (2014-2021).
Certificates and activities
* Data contributor in the project of Dental Diagnostics and Digital Dentistry Topic Group (TC-Dental) of the International Telecommunications Union (ITU) / World Health Organization (WHO) Focus Group on Artificial Intelligence for Health (FG-Al4H) (April 2023 till now)
*Certificate of participation for attending the course in (Online diagnostic seminars in oral pathology using digital slides (August 2021 – January 2022) held by (Digiscan).
*Certificate of Attendance in course of (Head and Neck and the Unknown, Dawn of a New year and a New World) on 7th Jan 2023 held by SGIAP-ASEAN Pathology.
* Member of Digital pathology association (DPA).
* Two posters contribution and presentations in the first Oral and Maxillofacial pathology scientific day /Cairo University (2019)
* International test of English as a foreign language (TOEFL – i.BT), (2009-2011)
* Student organizer in Egyptian dental association conferences (2003, 2005 and 2007)
*Head of Art committee in the student union (2005)
* Head of infection control committee in the quality of assurance & accreditation unit (2006 – 2007)
* Course in citizenship rights in the faculty of Economic & Political science / Cairo University.
Research:
- PhD thesis (Apoptotic and Anti-Proliferative Effects of Licorice Extract (Licochalcone A) and Paclitaxel Chemotherapy on Human Oral Squamous Cell Carcinoma Cell Line)
- Published paper in European Chemical Bulletin. (Anti-Proliferative Effects of Licochalcone A and Paclitaxel on Human Oral Squamous Cell Carcinoma Cell Line (In Vitro Study). (2023)
- Master thesis (IMMUNOHISTOCHEMICAL EXPRESSION OF PHOSPHATIDYL- INOSITOL-30 KINASE (PI3K)/AKT2 IN ORAL EPITHELIAL DYSPLASIA AND SQUAMOUS CELL CARCINOMA). (2014)
- Published paper in Egyptian dental journal. (IMMUNOHISTOCHEMICAL EXPRESSION OF PHOSPHATIDYL-INOSITOL-30 KINASE (PI3K)/AKT2 IN ORAL EPITHELIAL DYSPLASIA AND SQUAMOUS CELL CARCINOMA). (2015)
Training courses:
* Condensing course in (Online diagnostic seminars in oral pathology using virtual digital slides library (August 2021 – January 2022) held by (Digiscan).
* Elective course in teaching and learning strategies – faculty of dentistry- Cairo University. (2021)
* Elective course in dental photography in faculty of dentistry, Cairo University. (2019)
* Elective course in patient safety patient management, faculty of dentistry, Cairo university. (2019)
* Elective course in Nutrition – faculty of dentistry – Cairo University. (2018)
* General dental course in faculty of dentistry / Damascus university Syria through program of student substitute 2005 – 2006 / degree: Excellent.
Other knowledge & skills:
* Ability to use microscope camera in research work
* Very good in English written & spoken
* Professional skill in handwriting & calligraphy art.
* Ability to deal with various computer software's:
- Microsoft office - Internet browsing
Master Title
Immunohistochemical expression of phosphatidyl-inositol-30 kinase (PI3K)/Akt2 in oral epithelial dysplasia and squamous cell carcinoma
Master Abstract
Summary
Oral cancer is one of the most frequent cancers in the world and is thought to arise from progressive dysplastic changes of the oral squamous epithelium. The main purpose of identifying these lesions is to prevent the malignant transformation by initiating adequate intervention.
This study was performed to assess the cellular activity using AKT2 immunohistochemical expression in oral dysplastic lesions and OSCC.
Thirty-six paraffin blocks were investigated in this study including fifteen premalignant dyplastic lesions and fifteen OSCC. For each case, six sections from normal tissue as a control.
For immunohistochemical examination, the primary antibody used was monoclonal mouse anti-human Akt2 antibody of NOVUS biological (clone: X1, isotype: IgG1 Kappa).The universal DAKO labeled Streptavidin Biotin system + kit, horse radish peroxidase was also used.
The sections were analyzed by image analyzer computer system using the software Lecia Qwin 500.We measured the area percentage% of the positive nuclear and cytoplasimic immunoreactions in dysplastic and neoplastic cells.
Our findings revealed positive cytoplasmic and few nuclear immunoexpression Akt2 and it increases with increasing the stage of the lesion from mild dysplasia to OSCC.
The mean values of positivity were statistically studied using the (ANOVA) test to compare between mild, moderate-severe and severe dysplasia and well, moderately-poorly differentiated SCC while student's t-test was used to compare between mean values between premalignant and malignant lesions.
From the statistical analysis, it was found that area percentage% of Akt2 were extremely significant (p<0.0001) regarding to the premalignant and malignant lesions. Premalignant lesions including mild, moderate-severe also revealed significant results (p<0.0001) as compared to each others regarding to Akt2 immunoexpression. However, the area percentage of Akt2 regarding to different grades of SCC and was very significant (p<0.001).
It was concluded that Akt2 increased with the increase of different grades of oral epithelial dysplasia from mild dysplasia to OSCC and may also be an aid in the diagnosis and prognosis of these lesions and the subsequent response to the chemotherapeutic drugs.
PHD Title
Apoptotic and Anti-Proliferative Effects of Licorice Extract (Licochalcone A) and Paclitaxel Chemotherapy on Human Oral Squamous Cell Carcinoma Cell Line
PHD Abstract
SUMMARY
Oral squamous cell carcinoma is one of the main causes of death all over the world. Regarding World Health Organization (WHO) data, cancer is the second most common cause of death after cardiovascular diseases. Although the available curative clinical managements for oral squamous cell carcinoma, including surgical interventions, radiotherapy, and chemotherapy, only 50 – 60 % of patients have a 5–year survival rate.
This study was performed to assess the effect of Licochalcone A on cell viability, cell proliferation and apoptosis compared to Paclitaxel in cultured OSCC cell line (SCC-15) as well as to determine the role of Licochalcone A in enhancing the anti-proliferative and apoptosis inducing effects of Paclitaxel.
The cultured squamous cell carcinoma cell line (SCC-15) was obtained and divided into three treatment groups: Licochalcone A treated group, Paclitaxel treated group, combined drugs treatment group (Licochalcone A + Pacliatxel) and untreated control group.
For cell viability assessment, (SRB) assay was done by testing all treatment groups for 24, 48 and 72 hours after drug exposure in different concentrations. Regarding the immunofluorescent interpretation, Annexin V-FITC Apoptosis Staining / Detection Kit was obtained from Abcam company and was used to assess apoptosis in each treatment group. The fluorescent intensity was visualized under confocal fluorescent microscope.
For immunohistochemical examination, IPO-38 mouse monoclonal antibody proliferation marker purchased from Abcam company was used and the stained sections were examined under the light microscope. Mean value for area percent of proliferation marker IPO-38 immunohistochemical staining in the three treatment groups was measured by image analyzer computer system.
Our findings revealed that cell viability in Licochalcone A and Paclitaxel treatment groups was decreased in a concentration and time dependent manner compared to the control group. Additionally, the cell viability in combination treatment group showed more and obvious reduction in a concentration and time dependent manner.
Moreover, by comparison between all treatment groups over the longest time and highest concentrations, the least cell viability was seen in the combination group.
For immunohistochemical findings, comparing the studied groups by ANOVA test and according to the highest concentration for each treatment group among the longest duration (72 hours), the untreated control group of SCC-15 cells showed the highest area percent for IPO-38 immunoexpression while the area percent for IPO-38 decreased in the Licochalcone A group. The Paclitaxel and combination groups of SCC-15 cells showed the lowest area percent of IPO-38 immunoexpression. The P value was statistically highly significant for all compared groups.
Regarding the fluorescence microscopic findings and according to the ANOVA test, after 24, 48 and 72 hours at the highest concentrations, Annexin V immunofluorescence levels showed the highest values in the combination group followed by Paclitaxel group then LCA group, while the control group showed the least levels. The difference between the treatment modalities at each time was highly statistically significant.
According to our study, we concluded that the addition of Licochalcone A enhanced the effect of Paclitaxel on reducing cell proliferation and inducing apoptosis confirming the concept of having a synergistic effect during cancer treatment. This could be applied to reduce the well-known side effects of Paclitaxel on the cancer patients to improve their quality of life.