Basic Informations

C.V

Master Title

THE ASSESSMENT OF OSTEOPONTIN LEVEL IN GINGIVAL CREVICULAR FLUID IN PATIENT WITH PERIODONTAL DISEASE

Master Abstract

Periodontal diseases are chronic inflammatory diseases of the supporting structures of the teeth. Periodontal disease is triggered by periodontopathogens and the clinical outcome is highly influenced by the host local immune response. Human periodontal diseases result from heterogeneous etiologies that include a complex biofilm in the subgingival microenviroment, social and behavioral modulations, and genetic traits of the host, each of which is influenced by the host immune and inflammatory responses. Gingival crevicular fluid is inflammatory exudates that reflect continuing events in the periodontal tissue. Its value is increasing in the assessment of the biological state of the deeper-seated tissues of the periodontium and extensive search has been made for gingival crevicular fluid components that might serve as potential diagnostic or prognostic markers for the progression of periodontitis. OPN is produced by both osteoblasts and osteoclasts, it holds a dual function in bone maturation and mineralization as well as bone resorption. Several studies evaluated osteopontin level in human gingival crevicular fluid and found it to be correlated with periodontal disease presence and activity. The present study was conducted to evaluate to evaluate osteopontin levels in healthy and diseased periodontium and to possibly correlate the changes in its levels with the changes in the clinical parameters. Samples were collected from the gingival crevicular fluid of 20 healthy subjects and from the most periodontally affected sites of 30 subjects with chronic periodontitis and 30 subjects with aggressive periodontitis. The selection and grouping of the subjects was based on the classification of periodontal diseases and conditions in 1999. Filter paper was used in samples collection and the samples were analyzed using ELISA technique to evaluate the level of osteopontin among 3 studied group. Pocket depth, clinical attachment level, plaque index, gingival index and bone density were recorded to detect possible correlation with the levels of osteopontin in gingival crevicular fluid. Results revealed that osteopontin level was highest in aggressive periodontitis group followed by chronic periodontitis group followed by healthy (control) group. The statistical analysis showed that there was significant difference between aggressive periodontitis group and chronic periodontitis group concerning probing pocket depth, clinical attachment level, bone density, plaque index and gingival index. There was a significant correlation between osteopontin level, both pocket depth, clinical attachment level and bone density. The level of osteopontin was proportionally increased with pocket depth and clinical attachment level and inversely proportional with the bone density.

PHD Title

The effect of simvastatin gel in the treatment of intrabony defect in chronic periodontitis

PHD Abstract

Periodontal diseases are chronic inflammatory diseases of the supporting structures of the teeth. Periodontal disease is triggered by periodontopathogens and the clinical outcome is highly influenced by the host local immune response. Human periodontal diseases result from heterogeneous etiologies that include a complex biofilm in the subgingival microenviroment, social and behavioral modulations, and genetic traits of the host, each of which is influenced by the host immune and inflammatory responses. Simvastatin has been shown to inhibit bone resorption. However, this effect appears minor in comparison to its anabolic action on new bone formation and osteoblast maturation. Simvastatin has been found to halt bone loss in animals with induced periodontitis, acting as an anti-inflammatory and bone formation promoter. OPN is produced by both osteoblasts and osteoclasts, where it holds a dual function in bone maturation and mineralization as well as bone resorption. The present study was conducted to evaluate the effect of simvastatin gel in the treatment of intrabony defect in chronic periodontitis. The study was designed as split mouth in ten patients. Surgical procedure of open flap debridement was performed at affected sites to enable access to the defect. Simvastatin gel was delivered in ten sites and placement of placebo gel was done in ten sites. Samples of gingival crevicular fluid of 10 healthy subjects with chronic periodontitis were collected at base line, 3 and 6 months postsurgically. Filter paper was used in samples collection and the samples were analyzed using ELISA technique to evaluate the level of osteopontin on the studied group. Pocket depth, clinical attachment level, plaque index, gingival index, linear measurements and bone density were recorded to detect possible effect of simvastatin gel as regenerative periodontal therapy. Results revealed that in simvastatin sites gingival index, plaque index, pocket depth, attachment loss, linear and densitometric measurements were improved after 6 months compared with the base line. The statistical analysis showed that there was significant difference between a simvastatin group and placebo group in probing pocket depth, clinical attachment level, and bone density at the end of the study. The changes in the level of osteopontin were insignificant with simvastatin group at different follow up period. However it was significantly elevated in placebo group at 6 months.